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Related Concept Videos

Recombinant DNA01:09

Recombinant DNA

Overview
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
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Related Experiment Video

Updated: Jun 7, 2026

Homemade Site Directed Mutagenesis of Whole Plasmids
07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

Published on: May 11, 2009

DNA construction: homemade or ordered out?

Peter A Carr1

  • 1Center for Bits and Atoms and the Massachusetts Institute of Technology Media Lab, Massachusetts Institute of Technology, Cambridge, MA, USA. carr@media.mit.edu

Nature Methods
|October 30, 2010
PubMed
Summary
This summary is machine-generated.

Automated DNA construction significantly improves the creation of large DNA sequences. This optimization enhances efficiency for complex genetic engineering applications.

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Protocols for C-Brick DNA Standard Assembly Using Cpf1
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Automated Robotic Liquid Handling Assembly of Modular DNA Devices

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Related Experiment Videos

Last Updated: Jun 7, 2026

Homemade Site Directed Mutagenesis of Whole Plasmids
07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

Published on: May 11, 2009

Protocols for C-Brick DNA Standard Assembly Using Cpf1
12:03

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Published on: June 15, 2017

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • Biotechnology

Background:

  • Traditional DNA construction methods can be time-consuming and labor-intensive.
  • The demand for large, custom DNA sequences is increasing in various research fields.

Discussion:

  • Automation streamlines the assembly of DNA fragments, reducing manual errors.
  • Optimization strategies can improve yield and purity of constructed DNA molecules.

Key Insights:

  • Automated DNA construction enables rapid and reliable production of large target sequences.
  • This approach accelerates research in areas like gene synthesis and genome engineering.

Outlook:

  • Further integration of automation in DNA synthesis promises to lower costs and increase accessibility.
  • Future developments may focus on even larger and more complex DNA constructs.