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Updated: Jun 7, 2026

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Two-step stacking in capillary zone electrophoresis featuring sweeping and micelle to solvent stacking: I. Organic
1Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Hobart, Tasmania 7001, Australia. jquirino@utas.edu.au
Abstract:
Two-step stacking of organic cations by sweeping and micelle to solvent stacking (MSS) in capillary zone electrophoresis (CZE) is presented. The simple procedure involves hydrodynamic injection of a micellar sodium dodecyl sulfate solution before the sample that is prepared without the micelles. The micelles sweep and transport the cations to the boundary zone between the sample and CZE buffer. The presence of organic solvent in the CZE buffer induces the second stacking step of MSS. The LODs obtained for the four beta blocker and two tricyclic antidepressant test drugs were 20-50 times better compared to typical injection.
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