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Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers

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Lysozyme-imprinted polymer synthesized using UV free-radical polymerization.

Shuang Yu¹, Ai-Qin Luo, Dipti Biswal

¹School of Life Science, Beijing Institute of Technology, Beijing 100081, China; Center for Biomedical Engineering, University of Kentucky, Lexington, KY 40506, USA.

|November 2, 2010

Summary

Molecular imprinting successfully created a polymer that selectively binds lysozyme, a key protein. This novel method offers enhanced protein recognition for various applications.

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Area of Science:

Biomaterials Science
Polymer Chemistry
Analytical Chemistry

Background:

Molecular imprinting (MIP) fabricates polymers for selective molecular recognition, widely studied for small molecules.
Imprinting biological macromolecules like proteins is less common but crucial, with lysozyme being significant in food, pharmaceutical, and diagnostic fields.

Purpose of the Study:

To develop a molecularly imprinted polymer (MIP) capable of selectively recognizing and binding the protein lysozyme.
To evaluate the imprinting efficiency and specificity of the fabricated MIP for lysozyme compared to a non-imprinted polymer (NIP).

Main Methods:

Lysozyme MIP was synthesized using UV free-radical polymerization with lysozyme as the template, PEG600DMA as the cross-linking monomer, and methacrylic acid as the functional monomer.
Lysozyme was removed via enzymatic digestion to create binding sites, and a control NIP was synthesized without the template.
Binding specificity was assessed by exposing MIP and NIP to lysozyme, RNase A, or a mixture, followed by protein release via digestion.

Main Results:

The synthesized MIP demonstrated increased protein release with higher lysozyme concentrations, indicating more binding sites.
In competitive binding assays, MIP preferentially bound significantly more lysozyme (up to 20 times) than RNase A.
NIP showed minimal binding to both proteins and no preference, confirming the imprinting specificity of MIP.

Conclusions:

UV free-radical polymerization effectively imprinted lysozyme into the polymer matrix.
The fabricated lysozyme MIP exhibits high selectivity and preferential binding for its target protein, showing promise for applications in protein separation and sensing.