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Updated: Jun 7, 2026

Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
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Making and using calcium-selective mini- and microelectrodes.

L Hove-Madsen1, S Baudet, D M Bers

  • 1Cardiovascular Research Centre CSIC-ICCC, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.

Methods in Cell Biology
|November 2, 2010
PubMed
Summary
This summary is machine-generated.

Calcium-selective electrodes offer a complementary approach to fluorescent indicators for measuring intracellular calcium concentration. This chapter details the preparation and application of ETH 129-based electrodes for precise calcium measurements.

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Area of Science:

  • Biomedical Engineering
  • Cell Physiology
  • Analytical Chemistry

Background:

  • Intracellular calcium concentration ([Ca(2+)](i)) measurement is crucial in cell biology.
  • Traditional methods include Ca(2+)-selective electrodes and absorption indicators.
  • Fluorescent calcium probes (e.g., fura-2, indo-1, fluo-4) are widely used but have varying sensitivities.

Purpose of the Study:

  • To highlight the utility of Ca(2+)-selective electrodes as a complementary method to fluorescent indicators.
  • To describe the preparation and application of ETH 129-based Ca(2+)-selective minielectrodes.
  • To present the application of ETH 129-based Ca(2+)-selective microelectrodes (MEs) for intracellular calcium determination in cardiac cells.

Main Methods:

  • Preparation and application of Ca(2+)-selective minielectrodes utilizing the Ca(2+) ligand ETH 129.
  • Development and use of ETH 129-based Ca(2+)-selective microelectrodes (MEs).
  • Application of these electrodes for measuring intracellular calcium concentration ([Ca(2+)](i)) in cardiac cells.

Main Results:

  • ETH 129-based Ca(2+)-selective minielectrodes exhibit a larger dynamic range and faster response time compared to commercial electrodes.
  • Demonstrated successful application of ETH 129-based MEs for [Ca(2+)](i) determination in cardiac cells.
  • Highlighted the complementary nature of Ca(2+)-selective electrodes and fluorescent indicators, each with distinct advantages and disadvantages.

Conclusions:

  • Ca(2+)-selective electrodes, particularly ETH 129-based ones, provide valuable and complementary data for intracellular calcium measurements.
  • These electrodes offer advantages in dynamic range and response time for specific applications.
  • The chapter serves as a practical guide for researchers experienced with microelectrodes for [Ca(2+)](i) analysis.