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An Enhanced Green Fluorescence Protein-based Assay for Studying Neurite Outgrowth in Primary Neurons
Published on: October 19, 2019
Fluorescent quantitation method for differentiating the nativity of green fluorescent protein
Few Ne Chew1, Wen Siang Tan, Beng Ti Tey
1Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Journal of Bioscience and Bioengineering
|November 2, 2010
Abstract:
A gel imaging method was employed to quantitate the GFP that had been subjected to denaturation and degradation treatments. This method is able to differentiate the nativity of GFP by relating the observed changes in the position of fluorescent bands which is unable to be detected using the spectrofluorometric method.
