Related Experiment Video
Updated: Jun 7, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
Published on: July 5, 2016
Phase reconstruction and unwrapping from holographic interferograms of partially absorbent phase objects
Abstract:
A method for automated phase reconstruction from holographic interferograms of nonideal phase objects based on a two-dimensional Fourier transform is described. In particular, the problem of phase unwrapping is solved because earlier techniques are inappropriate for the phase unwrapping from interferograms of partially absorbent objects. A noise-level-dependent criterion for the binary mask that defines the unwrapping path for the flood algorithm is derived. The method shows high noise immunity, and the result is reliable provided that the true phase is free of discontinuities. The phase distribution in the outmasked regions is estimated by a linear least-squares fit to the surrounding unwrapped pixels.
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Reconstruction of Signal using Interpolation
Interference and Diffraction

