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Related Experiment Video

Updated: Jun 7, 2026

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

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Simple autogeneic feeder cell preparation for pluripotent stem cells.

Weizhen Li1, Hiromi Yamashita, Fumiyuki Hattori

  • 1Division of Cardiology, Department of Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

Stem Cell Research
|November 3, 2010
PubMed
Summary

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Autogeneic feeder (AF) cells, derived from the same species, can replace mouse embryonic fibroblasts (MEFs) for maintaining pluripotent stem cells. This approach eliminates xenogeneic risks and reduces costs while preserving stem cell pluripotency and differentiation potential.

Area of Science:

  • Stem Cell Biology
  • Cell Culture
  • Reproductive Medicine

Background:

  • Mouse embryonic fibroblasts (MEFs) are standard feeder cells for pluripotent stem cells.
  • Autogeneic feeder (AF) cells offer advantages like reduced xenogeneic risks and lower costs.

Purpose of the Study:

  • To evaluate the efficacy of autogeneic feeder cells for maintaining primate pluripotent stem cells.
  • To compare the performance of AF cells with MEFs in supporting stem cell cultures.

Main Methods:

  • Common marmoset embryonic stem (cmES) cells were cultured on common marmoset AF (cmAF) cells.
  • Human embryonic stem (hES) and induced pluripotent stem (hiPS) cells were cultured on their respective AF cells.
  • Cell morphology, karyotype, pluripotent marker expression, and differentiation potential were assessed.

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  • Protein and mRNA expression profiles were analyzed using antibody arrays and DNA microarrays.

    • Main Results:

    • cmES cells maintained on cmAF cells for 6 months retained normal morphology, karyotype, and pluripotent marker expression.
    • These cmES cells differentiated into cardiac and neural lineages.
    • Protein expression profiles of cmES cells on cmAF cells were equivalent to those on MEFs.
    • hES and hiPS cells cultured on their respective AF cells showed similar morphology and marker expression compared to those on MEFs.
    • mRNA expression profiles of hES and hiPS cells on AF cells mirrored those on MEFs.

    Conclusions:

    • Autogeneic feeder cells can effectively replace MEFs for the routine maintenance of primate pluripotent stem cells.
    • This finding supports the use of species-specific feeder cells to enhance the safety and efficiency of stem cell culture.
    • AF cells provide a viable alternative for pluripotent stem cell culture, minimizing risks associated with xenogeneic materials.