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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
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Related Experiment Video

Updated: Jun 7, 2026

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Array tomography: immunostaining and antibody elution.

Kristina D Micheva, Nancy O'Rourke, Brad Busse

    Cold Spring Harbor Protocols
    |November 3, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Array tomography uses serial sectioning for 3D microscopy. This method enables detailed visualization and quantification of tissue architecture and molecular structures.

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    Area of Science:

    • Biotechnology
    • Microscopy
    • Cell Biology

    Background:

    • Array tomography is a volumetric microscopy technique.
    • It relies on physical serial sectioning of embedded tissues.

    Purpose of the Study:

    • To detail the protocol for preparing tissue arrays for imaging.
    • To enable high-resolution 3D visualization and quantitative analysis of tissue structures.

    Main Methods:

    • Physical serial sectioning of plastic-embedded tissue using an ultramicrotome.
    • Bonding ultrathin sections into an ordered array on a coverslip.
    • Staining, imaging, and computational reconstruction into 3D volumes.
    • Optional: Repeated staining/imaging cycles and multimodal imaging (fluorescence, electron microscopy).

    Main Results:

    • Enables visualization and quantification of previously inaccessible tissue and molecular architecture features.
    • High-quality, rapid staining and imaging due to minimal section thickness.
    • Reliable section handling, staining, and automated imaging via array format.

    Conclusions:

    • Array tomography is a powerful method for detailed 3D tissue analysis.
    • Careful tissue preparation is crucial for successful implementation.
    • The protocol allows for versatile imaging modalities and repeated analysis cycles.