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Related Concept Videos

Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: Jun 7, 2026

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

Array tomography: semiautomated image alignment.

Kristina D Micheva, Nancy O'Rourke, Brad Busse

    Cold Spring Harbor Protocols
    |November 3, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Array tomography enables high-resolution 3D visualization of tissue structure by physically sectioning, staining, and imaging samples. This method allows detailed analysis of molecular architecture previously inaccessible with other microscopy techniques.

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    Area of Science:

    • Microscopy
    • Biotechnology
    • Cell Biology

    Background:

    • Array tomography is a volumetric microscopy technique.
    • It relies on physical serial sectioning of plastic-embedded tissues.
    • This method enables high-resolution 3D imaging and analysis.

    Purpose of the Study:

    • To describe the protocol for array tomography.
    • To enable 3D visualization and analysis of tissue structure and molecular architecture.
    • To facilitate the construction of volumetric image stacks from fluorescently labeled arrays.

    Main Methods:

    • Physical serial sectioning of plastic-embedded tissue using an ultramicrotome.
    • Bonding ultrathin sections into an ordered array on a glass coverslip.
    • Staining, imaging, and computational reconstruction of 2D image tiles into 3D volumes.

    Main Results:

    • Achieved high-quality rapid staining and imaging due to minimal section thickness.
    • Enabled reliable and convenient section handling, staining, and automated imaging via the array format.
    • Facilitated repeated cycles of staining, imaging, and stain elution for multi-modal imaging.

    Conclusions:

    • Array tomography provides unprecedented visualization and quantification of tissue structure and molecular architecture.
    • Careful tissue preparation and image alignment are crucial for successful implementation.
    • Freely available software supports the construction of 3D image stacks for analysis and archiving.