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Updated: Jun 7, 2026

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
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Single cell deposition and patterning with a robotic system.

Zhe Lu1, Christopher Moraes, George Ye

  • 1Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, Ontario, Canada.

Plos One
|November 3, 2010
PubMed
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A new robotic system precisely positions single cells for biological studies. This automated pick-and-place technology enhances cell culture and single-cell analysis efficiency.

Area of Science:

  • Biotechnology
  • Robotics
  • Cell Biology

Background:

  • Integrating single-cell manipulation into biological cultures is challenging.
  • Microfabricated devices require precise spatial positioning of cells.
  • Existing methods for single-cell handling have drawbacks.

Purpose of the Study:

  • To develop a robotic micromanipulation system for accurate pick-and-place positioning of single cells.
  • To investigate different cell aspiration techniques for optimal manipulation.
  • To demonstrate the system's applicability across various cell types and substrates.

Main Methods:

  • A robotic micromanipulation system integrating computer vision and motion control was developed.
  • Traditional glass micropipettes were used with whole- and partial-cell aspiration techniques.

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Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
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Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

Published on: March 7, 2014

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Last Updated: Jun 7, 2026

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
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Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells
10:14

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Published on: November 18, 2016

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
07:19

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

Published on: March 7, 2014

  • The system performs real-time cell tracking, picking, transferring, and depositing.

    • Main Results:

    • Partial-cell aspiration achieved 95% success rate in 15 seconds per cell.
    • Whole-cell aspiration yielded an 80% success rate in 30 seconds per cell.
    • The system demonstrated broad applicability on diverse cell types and microfabricated devices, improving single-cell capture efficiency from ~80% to 100% when combined with parallel techniques.

    Conclusions:

    • Robotic micromanipulation is an effective technology for precise single-cell positioning.
    • This system offers a solution to challenges in single-cell handling and manipulation.
    • The technology can be used independently or to enhance existing single-cell analysis methods.