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Updated: Jun 7, 2026

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
Published on: June 5, 2019
Comparative evaluation of PAR1, GPIb-IX-V, and integrin αIIbβ3 levels in cord and adult platelets
A Schlagenhauf1, S Schweintzger, R Birner-Grünberger
1Department of Pediatrics, Medical University of Graz, Auenbruggerplatz 30, 8036 Graz, Austria. wolfgang.muntean@meduni-graz.at
Background:
Newborn platelets show hyposensitivity in vitro to many important agonists. However, sensitivity of platelets to these agonists is crucial for a functional clot formation. Nevertheless newborns have an excellent hemostasis. Hence, we examined levels of PAR1 thrombin receptor, GPIb-IX-V (CD42b), and Integrin αIIbβ3 in newborn and adult platelets using Western blot analysis. Materials, methods: Platelets of adult and cord blood were isolated, washed, and lysed. Protein samples were separated by SDS-PAGE and blotted on nitrocellulose membranes. Receptors were visualized using immunodetection and evaluated densitometrically. Statistical analysis was performed using SPSS 16.0.
Results:
We found significantly lower levels of PAR1-receptors and higher levels of CD42b in newborn platelets as compared to adult platelets. Levels of Integrin αIIbβ3 in newborn platelets were comparable to adult platelets.
Conclusion:
A lower content of PAR1-receptors explains very well the hyposensitivity of cord platelets to thrombin. Higher levels of CD42b may additionally support the effect of larger more adhesive multimeric vWF in newborn plasma.
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