Related Experiment Video
Updated: Jun 7, 2026
Blood Sampling and Hormone Measurement for Determining the Stage in the Ovarian Cycle in Marmosets
Published on: July 5, 2024
Molecular cloning and functional characterization of endogenous recombinant common marmoset monkey (Callithrix
T Müller1, T Hupfeld, J Roessler
1Stem Cell Biology Unit, German Primate Center, Göttingen, Germany. mueller.thomas@mh-hannover.de
Background:
Common marmoset monkeys (Callithrix jacchus) are readily used in biomedical research. However, superovulation for embryonic stem cell production and developmental research still remain difficult. Inexplicably, follicle-stimulating hormone (FSH) as key player in superovulation has to be administered in extremely high dosages in this non-human primate compared to human.
Methods:
To evaluate whether marmoset FSH (cjFSH) is functionally more competent than its human homologue on the marmoset FSH receptor (cjFSHR), we established in vitro a homologous system characterizing homologous and recombinantly produced cjFSH.
Results:
Upon stimulation of two cell lines stably expressing either the marmoset or the human FSH receptor (cj/hFSHR), respectively, the second messenger signaling of the activated receptor displayed no significant difference in ED(50) values. Thermostability of cjFSH was significantly prolonged by roughly 20% on both FSHRs.
Conclusion:
High FSH dosage in marmoset superovulation cannot be explained by enhanced biopotency of the natural animal's gonadotropin.
Related Concept Videos
01:08Cloning of Dolly the Sheep
01:27Reproductive Cloning
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
01:31Hybridoma Technology
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...