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Related Concept Videos

Enzymes02:34

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Introduction To Enzymes01:22

Introduction To Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...
Introduction to Enzymes01:22

Introduction to Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...

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Related Experiment Video

Updated: Jun 7, 2026

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
11:27

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

Published on: May 13, 2020

ThYme: a database for thioester-active enzymes.

David C Cantu1, Yingfei Chen, Matthew L Lemons

  • 1Department of Chemical and Biological Engineering, Iowa State University, Ames, Iowa 50011, USA.

Nucleic Acids Research
|November 4, 2010
PubMed
Summary
This summary is machine-generated.

The ThYme database consolidates enzyme sequences and structures involved in fatty acid and polyketide synthesis. This resource aids research into thioester-active enzymes and their evolutionary relationships.

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Last Updated: Jun 7, 2026

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Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

Area of Science:

  • Biochemistry
  • Structural Biology
  • Bioinformatics

Background:

  • Fatty acid and polyketide synthesis involve complex enzyme machinery.
  • Thioester-active enzymes play crucial roles in these pathways.
  • Understanding enzyme structure and sequence is key to elucidating function.

Purpose of the Study:

  • To create a centralized database of enzymes involved in fatty acid and polyketide synthesis.
  • To compile amino acid sequences and 3D structures of these enzymes.
  • To facilitate research on thioester-active enzymes and their families.

Main Methods:

  • Database construction integrating sequence and structural data.
  • Classification of enzymes into families based on sequence, structure, and mechanism.
  • Continuous updates with newly available data.

Main Results:

  • The ThYme database is established, housing data for key enzyme groups.
  • Enzymes are categorized into families suggesting common protein ancestry.
  • The database includes acyl-CoA synthase, carboxylase, transferase, ketoacyl synthase, reductase, hydroxyacyl dehydratase, enoyl reductase, and thioesterase groups.

Conclusions:

  • The ThYme database provides a valuable resource for studying fatty acid and polyketide synthesis enzymes.
  • Enzyme family classification highlights evolutionary connections.
  • The database supports ongoing research by offering updated sequence and structural information.