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Updated: Jun 7, 2026

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
Published on: March 19, 2020
Immobilization of Thermoanaerobium brockii alcohol dehydrogenase on SBA-15
Michela Vittorini1, Emil Dumitriu, Gabriel Barletta
1Istituto di Chimica del Riconoscimento Molecolare, Consiglio Nazionale delle Ricerche, via M. Bianco 9, Milan, Italy.
Abstract:
The activity of Thermoanaerobium brockii alcohol dehydrogenase (TBADH) adsorbed on mesoporous silica SBA-15 was compared with that of the free enzyme in water and in biphasic system (water phase up to 50% v/v water). TBADH was active at a water concentration ≥10% v/v. In the reduction reaction of sulcatone to sulcatol carried out in biphasic systems, the yield obtained with SBA-15-adsorbed TBADH was up to 5.5-fold higher than that with the free enzyme, which suggests a higher stability of the immobilized enzyme toward the organic solvent. The nature of the organic solvent substantially influenced the degree of conversion that, for example, was 7.4% in toluene and 31.6% in petroleum ether.
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