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Polymorphonuclear leukocytes induced vasoconstriction in isolated canine coronary arteries
M Nishida1, T Kuzuya, S Hoshida
1First Department of Medicine, Osaka University School of Medicine, Japan.
Circulation Research
|January 1, 1990
Summary
Polymorphonuclear leukocytes (PMNs) induce coronary artery contraction by releasing vasoconstrictive substances through metabolic interactions with endothelial cells. This process involves 5-lipoxygenase metabolites and contributes to vascular smooth muscle contraction.
Area of Science:
- Cardiovascular Physiology
- Immunology
- Cellular Biology
Background:
- Polymorphonuclear leukocytes (PMNs) play a role in inflammatory processes.
- The impact of PMNs on coronary vasoactivity requires further elucidation.
Purpose of the Study:
- To investigate the effect of PMNs on coronary artery tension.
- To understand the mechanism of PMN-induced coronary vasoconstriction.
Main Methods:
- Isometric tension measurements of isolated canine coronary artery rings.
- Isolation of PMNs using Percoll gradient.
- Assessment of endothelial cell integrity and function.
- Inhibition of 5-lipoxygenase pathway.
Main Results:
- PMNs induced dose-dependent isometric tension in coronary artery rings.
- PMN-induced contraction was endothelium-dependent and reversible by acetylcholine.
- Mechanical removal of endothelium abolished PMN-induced vasoconstriction.
- 5-lipoxygenase inhibitors significantly suppressed PMN-induced vasoconstriction.
Conclusions:
- PMNs induce contraction of coronary arteries via intact endothelial cells.
- The mechanism involves the release of vasoconstrictive substances, likely 5-lipoxygenase metabolites, through PMN-endothelial cell metabolic interactions.
- This PMN-endothelial system may contribute to vascular smooth muscle contraction.