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Related Concept Videos

Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...

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Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
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[Gene therapy with vector-producing multipotent mesenchymal stromal cells].

Takashi Okada1

  • 1Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan. t-okada@ncnp.go.jp

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
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Mesenchymal stem cells (MSCs) carrying retroviral vectors effectively target and treat glioma in mice. This approach enhances suicide gene therapy for recurrent glioblastoma by improving vector delivery to tumor cells.

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Area of Science:

  • Oncology
  • Gene Therapy
  • Stem Cell Biology

Background:

  • Recurrent glioblastoma poses treatment challenges, with limited efficacy of current therapies.
  • Suicide gene therapy shows promise but requires improved vector delivery to target tumor cells.
  • Mesenchymal stem cells (MSCs) are potential delivery vehicles for cancer therapies due to their tumor-homing abilities.

Purpose of the Study:

  • To develop and evaluate vector-producing MSCs for enhanced suicide gene therapy against glioma.
  • To investigate the efficacy of MSC-mediated gene delivery and tumor transduction in vivo.

Main Methods:

  • Rodent MSCs were engineered to produce retroviral vectors using nucleofection.
  • Vector-producing MSCs were administered intravenously to athymic nude mice bearing subcutaneous 9L glioma.
  • Optical bioluminescence imaging tracked MSC accumulation in tumors.
  • Therapeutic efficacy was assessed by measuring pro-drug mediated killing of glioma cells.

Main Results:

  • Vector-producing MSCs selectively accumulated in subcutaneous 9L glioma tumors.
  • MSC-mediated delivery significantly enhanced pro-drug killing of glioma cells compared to control MSCs.
  • The study demonstrated effective MSC-mediated tumor transduction with progeny vector production.

Conclusions:

  • MSCs can serve as effective delivery vehicles for suicide gene therapy, improving vector transduction in tumors.
  • This MSC-mediated strategy holds potential for eradicating evasive tumors in situ.
  • Further validation in orthotopic and metastatic models is warranted to confirm therapeutic benefits.