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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Cell-based screening using high-throughput flow cytometry.

Christopher B Black1, Thomas D Duensing, Linda S Trinkle

  • 1IntelliCyt Corporation, 317 Commercial St. NE, Albuquerque, NM 87102, USA. cblack@intellicyt.com

Assay and Drug Development Technologies
|November 6, 2010
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Summary

High-throughput flow cytometry (HTFC) enables rapid, multiplexed cell and bead screening. Innovations in instrumentation, like the HTFC Screening System, overcome previous limitations for diverse biological applications.

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Area of Science:

  • Biotechnology
  • Cellular Biology
  • Assay Development

Background:

  • Flow cytometry was historically underutilized for large-scale screening due to sample handling limitations.
  • Recent innovations in instrumentation and increasing demand for cell-based assays have led to a resurgence in its use.
  • High-throughput flow cytometry (HTFC) offers a powerful solution for multiplexed screening.

Purpose of the Study:

  • To review the application of high-throughput flow cytometry for multiplexed cell-based and bead-based screening.
  • To highlight the capabilities of the HTFC™ Screening System, including its fast sample loading and data analysis features.
  • To showcase the versatility of HTFC in various biological assay areas.

Main Methods:

  • Utilized the HTFC™ Screening System, featuring HyperCyt® fast sample-loading technology and a two-laser, six-parameter flow cytometer.
  • Employed HyperView® data analysis software for rapid hit identification in multiplexed screening campaigns.
  • Leveraged a server-based data management platform for enhanced data accessibility and collaboration.

Main Results:

  • The HTFC system achieves screening speeds of up to 40 wells per minute.
  • Processing of 96- and 384-well plates is possible in as little as 3 and 12 minutes, respectively.
  • Demonstrated successful application in diverse areas including transporter assays, receptor binding, GPCR screening, toxicology, and antibody screening.

Conclusions:

  • High-throughput flow cytometry, particularly with the HyperCyt technology, is a viable and efficient tool for multiplexed screening.
  • The described system facilitates rapid analysis and seamless data management for large-scale screening campaigns.
  • HTFC is applicable across a wide range of biological research and drug discovery applications.