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Galactose inhibits lactase expression by mouse jejunal enterocytes
M W Smith1, P S James, A J Collins
1A.F.R.C. Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, U.K.
Biochimica Et Biophysica Acta
|January 23, 1990
Summary
Galactose consumption in mice reduces enterocyte migration and lactase expression, contrasting with previous findings in rats. This suggests galactose metabolism impacts intestinal enzyme activity differently across species.
Area of Science:
- Gastroenterology
- Cell Biology
- Nutritional Science
Background:
- Galactose metabolism and its impact on intestinal enzyme expression are not fully understood.
- Previous studies indicated galactose increases lactase biosynthesis in rat intestine.
Purpose of the Study:
- To investigate the effects of galactose on lactase expression in mouse enterocytes.
- To compare galactose's impact on enterocyte migration and enzyme activity in mice.
Main Methods:
- Quantitative cytochemistry was used to measure lactase expression.
- Cell migration rates of enterocytes were measured in mice fed galactose versus a control diet.
- Sucrase-maltase activity was measured as a control.
Main Results:
- Galactose-fed mice exhibited reduced food intake, body weight, and enterocyte migration rate.
- Lactase activity increased more rapidly but persisted for a shorter duration in low-carbohydrate-fed mice compared to galactose-fed mice.
- Galactose feeding did not affect sucrase-maltase activity.
Conclusions:
- Galactose affects lactase expression in mouse enterocytes, potentially due to metabolic limitations.
- Findings in mice contradict previous reports in rats, highlighting species-specific responses.
- Enterocyte age and developmental stage are critical factors in studying enzyme expression.