Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A plasmid vector with a selectable marker for halophilic archaebacteria.

M L Holmes¹, M L Dyall-Smith

¹Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.

Journal of Bacteriology

|February 1, 1990

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An improved reprogrammable mouse model harbouring the reverse tetracycline-controlled transcriptional transactivator 3.

Stem cell research·2016

Same author

BCL-2 is dispensable for thrombopoiesis and platelet survival.

Cell death & disease·2015

Same author

Combined use of cultivation-dependent and cultivation-independent methods indicates that members of most haloarchaeal groups in an Australian crystallizer pond are cultivable.

Applied and environmental microbiology·2004

Same author

Bacteremia due to Leptotrichia trevisanii sp. nov.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2002

Same author

Inducible expression of the megakaryocyte-specific gene glycoprotein IX is mediated through an Ets binding site and involves upstream activation of extracellular signal-regulated kinase.

Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research·2001

Same author

A new simvastatin (mevinolin)-resistance marker from Haloarcula hispanica and a new Haloferax volcanii strain cured of plasmid pHV2.

Microbiology (Reading, England)·2001

Researchers developed a novel genetic system for halophilic archaea using a novobiocin resistance gene. This system enables efficient gene transfer and analysis in Haloferax species, advancing archaeal genetics.

Area of Science:

Microbiology
Genetics
Archaea

Background:

Halophilic archaea, such as Haloferax, are extremophilic microorganisms with unique biological properties.
Genetic manipulation systems for archaea are crucial for understanding their biology and potential applications.

Purpose of the Study:

To develop a novel genetic transformation system for halophilic archaea.
To isolate and characterize a novobiocin resistance gene for use as a selectable marker.

Main Methods:

Selection of a novobiocin-resistant mutant from Haloferax.
Isolation of the resistance gene on a DNA fragment.
Cloning the gene into a plasmid (pHK2).
Transformation of wild-type Haloferax and Halobacterium volcanii.

Related Experiment Videos

Main Results:

A novobiocin resistance gene was identified and cloned.
The recombinant plasmid efficiently transformed wild-type cells and was stably maintained.
The system demonstrated high transformation efficiency (greater than 10^6/micrograms).
The system successfully transformed Halobacterium volcanii.

Conclusions:

A functional genetic transformation system for halophilic archaea was established.
This system facilitates genetic analysis in Haloferax and related species.
The novobiocin resistance marker provides a valuable tool for archaeal genetics research.