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Gas Chromatography: Types of Columns and Stationary Phases

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Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach

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Published on: December 4, 2021

High performance stationary phases for planar chromatography.

Salwa K Poole¹, Colin F Poole

¹Detroit District Laboratory, US Food and Drug Administration, 300 River Place, Suite 5900, Detroit, MI 48207, USA.

Journal of Chromatography. A

|November 9, 2010

Summary

This review examines thin-layer chromatography performance, highlighting forced flow and electrochromatography for stabilized particle layers. Improvements in instrumentation are needed for ultra-thin-layer chromatography systems.

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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

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Published on: April 26, 2016

Area of Science:

Analytical Chemistry
Separation Science

Background:

Review of kinetic performance for stabilized particle layers, particle membranes, and thin films in thin-layer chromatography (TLC).
Focus on factors influencing plate height, including layer characteristics and experimental conditions.

Purpose of the Study:

Identify optimal chromatographic techniques for stabilized particle layers.
Analyze band broadening mechanisms in TLC under various flow conditions.
Explore potential improvements for TLC media and instrumentation.

Main Methods:

Literature review of kinetic performance in TLC.
Analysis of band broadening mechanisms (molecular diffusion, mass transfer, flow anisotropy).
Evaluation of different flow systems (capillary, forced flow, pressurized planar electrochromatography).

Main Results:

Forced flow and pressurized planar electrochromatography show promise for stabilized particle layers, surpassing capillary flow limitations.
Band broadening in conventional/high-performance TLC is governed by molecular diffusion at low velocities and mass transfer/flow anisotropy at higher velocities.
Limited structural modifications can enhance stabilized particle layers for capillary flow; forced flow offers more avenues for improvement.

Conclusions:

Forced flow and electrochromatography are key for advancing stabilized particle layer performance in TLC.
Ultra-thin-layer chromatography media present opportunities for miniaturized systems, pending instrumentation advancements in sample application and detection.