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Updated: Jun 6, 2026

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans
08:10

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Published on: February 23, 2019

A New Approach for Measuring Single-Cell Oxygen Consumption Rates.

Timothy W Molter1, Sarah C McQuaide, Mark R Holl

  • 1Department of Electrical Engineering, University of Washington, Seattle, WA 98195-2500 USA ( tmolter@washington.edu ; smcquaid@washington.edu ).

IEEE Transactions on Automation Science and Engineering : a Publication of the IEEE Robotics and Automation Society
|November 9, 2010
PubMed
Summary

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A new system measures single-cell oxygen consumption rates using specialized microwells and oxygen sensors. This technology accurately quantifies cellular metabolic activity, crucial for understanding cell function and disease.

Area of Science:

  • Biotechnology
  • Cellular Biology
  • Biomedical Engineering

Background:

  • Understanding cellular metabolism is vital for disease research.
  • Existing methods for measuring oxygen consumption rates often lack single-cell resolution.

Purpose of the Study:

  • To present a novel system for precise measurement of single-cell oxygen consumption rates.
  • To validate the system's accuracy using human epithelial lung cancer cells.

Main Methods:

  • Development of a system with a temperature-controlled chamber, microwells, and oxygen-sensitive sensors.
  • Utilizing custom automation software for data collection and image-processing for analysis.
  • Measuring oxygen concentration over time within individual microwells.

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Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
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Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Published on: February 3, 2023

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Last Updated: Jun 6, 2026

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans
08:10

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Published on: February 23, 2019

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry
09:16

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Published on: February 3, 2023

Main Results:

  • The novel system successfully measured single-cell oxygen consumption rates.
  • Measurements for A549 cells were 5.39 and 5.27 fmol/min/cell.
  • Results closely aligned with published bulk population data for A549 cells.

Conclusions:

  • The developed system provides accurate single-cell metabolic rate measurements.
  • This technology offers a valuable tool for cellular metabolism research.
  • The system's precision supports further investigation into cellular function and disease.