Related Experiment Video
Updated: Jun 6, 2026
Lung Tumor Cell Recruitment Assay
Published on: February 26, 2019
[HGPRT-defective Lewis lung carcinoma cell line and its biological characteristics]
1Cancer Center and Cancer I nstitute, West China Hospital, Sichuan University ( Former The First University Hospital of West China University of Medical Sciences) , Chengdu , Sichuan 610041, P. R. China.
Background:
To provide an HGPRT ( hypoxanthine guanidine phosphoribosyl transferase)-defective cell line for the establishment of lung cancer and dendritic cell( DC) fused vaccine.
Methods:
The HGPRT-defective cell line was gradually induced by 8-AG ( 8-azaguanine) from the Lewis lung carcinoma cell line( L3-8) . Its biological characteristics and tumorigenicity were observed in vivo and in vitro.
Results:
The HGPRT-defective cell line, AL9901, was obtained after one year of selective culture and identification. AL9901 cell line could grow steadily in 20mg/ L 8-AG, but not in HAT selective medium. The chromosome modal numbers of AL9901 and L3-8 cell line were 58 and 62 respectively, lung metastatic rates were 30%( 3/ 10) and 70%( 7/ 10) respectively, and their tumorigenic rates were both 100%( 10/ 10) .
Conclusions:
The HGPRT-defective cell line, AL9901, maintains the biolog ical characteristics and carcinogenicity of the L3-8 cell line. It can be used as a parental antigenic cell for the establishment of Lewis lung carcinoma-DC fused tumor vaccine.
More Related Videos
09:45Generation of 3D Whole Lung Organoids from Induced Pluripotent Stem Cells for Modeling Lung Developmental Biology and Disease
Published on: April 12, 2021
09:02Pre-Conditioning the Airways of Mice with Bleomycin Increases the Efficiency of Orthotopic Lung Cancer Cell Engraftment
Published on: June 28, 2018