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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 6, 2026

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
07:48

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging

Published on: June 7, 2014

Mirror-attenuator-mosaic diffuser performance.

J Mueller, W Preuss, A Schirmacher

    Applied Optics
    |November 10, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Mirror-attenuator-mosaic diffusers provide consistent diffuse radiance regardless of incident light angle. Diamond drilling is a superior production technique compared to etching for these calibration sources.

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    Area of Science:

    • Optical engineering
    • Radiometry
    • Materials science

    Background:

    • Diffuse radiance sources are crucial for calibrating radiometers.
    • Mirror-attenuator-mosaic diffusers offer angle-independent output.
    • Existing production methods for diffusers require optimization.

    Purpose of the Study:

    • To compare the performance of diffusers produced by etching and diamond drilling.
    • To introduce a novel measurement setup for evaluating diffuser performance.
    • To determine the optimal technique for producing directional diffusers.

    Main Methods:

    • Developed a new measurement setup for comparing incident and outgoing light.
    • Achieved measurement accuracy better than 0.5% using the new setup.
    • Fabricated directional diffusers using both etching and diamond drilling techniques.

    Main Results:

    • Diffuse radiance output was found to be largely independent of incident light angle.
    • The diamond drilling technique demonstrated significantly better performance than etching.
    • The new measurement setup enabled precise evaluation of diffuser characteristics.

    Conclusions:

    • Diamond drilling is the preferred method for producing high-performance directional diffusers.
    • The developed measurement system is suitable for accurate calibration source evaluation.
    • Angle-independent diffuse radiance from these diffusers is valuable for radiometer calibration.