Related Experiment Video
Updated: Jun 6, 2026
Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
Published on: November 6, 2021
Use of primary culture of human fibroblasts in gingiva augmentation procedure
Marzena Dominiak1, Jolanta Saczko, Hanna Gerber
1Department of Dental Surgery, Silesian Piast Medical University, Wrocław, Poland.
Background:
The aim of this study was to assess gingival aesthetic after usage of an own method of primary culture of human fibroblasts derived from the connective tissue of oral cavity keratinized gingiva on collagenous carrier in gingival augmentation procedures.
Materials And Methods:
Procedures were performed on 10 patients (7 females, 3 males) aged 18-35 years. In total, 34 teeth in the anterior part of the maxilla and the mandible underwent treatment. The protocol consisted of (1) preparing the patient for tissue biopsy, (2) biopsy of keratinized tissue, (3) laboratory tissue culture, (4) application of expanded cells into the recipient site, and (5) post-procedure management. Aesthetic index, pocket depth (PD), and plaque (PI1) and after surgery complications were examined.
Results:
There was post-procedure aesthetic improvement in all 34 cases compared with the pre-procedure condition (grade 1), and furthermore a significant decrease in PD and PI1 were revealed.
Conclusion:
Use of own method of primary culture of human fibroblasts on a collagenous carrier for gingival augmentation is an aesthetic method of mucogingival complex repair.
