Related Experiment Video
Updated: Jun 6, 2026
Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation
Published on: January 10, 2025
Hesperidin, a flavanone glycoside, on lipid peroxidation and antioxidant status in experimental myocardial ischemic
Palanisamy Selvaraj1, Kodukkur Viswanathan Pugalendi
1Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.
Insights
Hesperidin demonstrated cardioprotective effects against myocardial ischemia in rats. This natural compound reduced cardiac damage markers and improved antioxidant levels, suggesting therapeutic potential for heart conditions.
Area of Science:
- Cardiology
- Pharmacology
- Biochemistry
Background:
- Myocardial infarction is a major global cause of death.
- Effective treatments for myocardial ischemia are urgently needed.
- Hesperidin, a flavonoid, is being investigated for its therapeutic potential.
Purpose of the Study:
- To evaluate the cardioprotective effects of hesperidin against isoproterenol-induced myocardial ischemia in a rat model.
- To assess the impact of hesperidin on cardiac biomarkers and oxidative stress parameters.
Main Methods:
- Myocardial ischemia was induced in rats using isoproterenol hydrochloride.
- Hesperidin was administered at doses of 100, 200, and 400 mg/kg.
- Cardiac markers, lipid peroxidation, and antioxidant enzyme activities were measured.
Main Results:
- Isoproterenol administration significantly elevated cardiac markers and lipid peroxidation while decreasing antioxidant levels.
- Hesperidin treatment, particularly at 200 and 400 mg/kg, significantly reduced cardiac markers and lipid peroxidation.
- Hesperidin treatment restored antioxidant levels and enzyme activities in the ischemic hearts.
Conclusions:
- Hesperidin exhibits significant cardioprotective effects in an experimental model of myocardial ischemia.
- The cardioprotective mechanism of hesperidin involves its potent antioxidant and anti-lipid peroxidative properties.
- Hesperidin represents a promising therapeutic agent for managing myocardial ischemia and related cardiovascular conditions.
Abstract:
Myocardial infarction continues to be a leading cause of mortality world-wide. Novel therapies are needed to treat the myocardial ischemia. This study was undertaken to evaluate the cardioprotective role of hesperidin on isoproterenol-induced myocardial ischemia in rats. Myocardial ischemia was induced by subcutaneous injection of isoproterenol hydrochloride (85 mg/kg body weight), for two consecutive days. Isoproterenol-administered rats showed elevated levels of cardiac markers (aspartate transaminase, alanine transaminase, lactate dehydrogenase, creatine kinase, creatine kinase-MB, cardiac troponins T and I) when compared with control and hesperidin treatment groups (100, 200 and 400 mg/kg body weight). The serum levels of cardiac markers were significantly reduced at the doses of 200 mg and 400 mg. All further experiments were carried out at the 200 mg dose. Lipid peroxidation markers (thiobarbituric acid reactive substances, lipid hydroperoxides and conjugated dienes) were elevated significantly in the plasma and heart whereas non-enzymic antioxidants (vitamin C, vitamin E and reduced glutathione) were decreased significantly. Activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase declined significantly in the heart of ischemic rats. However, after hesperidin treatment, all the above parameters reverted to normal levels. This study demonstrated that the cardioprotective effect of hesperidin on ischemic rats could be due to its anti-lipid peroxidative and antioxidant properties.
