Related Experiment Video

Updated: Jun 6, 2026

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
05:47

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

An electrospray/inductively coupled plasma dual-source time-of-flight mass spectrometer for rapid metallomic and

Duane A Rogers, Steven J Ray, Gary M Hieftje

    Metallomics : Integrated Biometal Science
    |November 12, 2010
    PubMed

    More Related Videos

    Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics09:18

    Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

    Published on: April 17, 2017

    Related Concept Videos

    Tandem Mass Spectrometry01:21

    Tandem Mass Spectrometry

    Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
    MALDI-TOF Mass Spectrometry01:19

    MALDI-TOF Mass Spectrometry

    Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
    Mass Spectrum: Interpretation01:24

    Mass Spectrum: Interpretation

    An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
    Electrospray Ionization (ESI) Mass Spectrometry01:12

    Electrospray Ionization (ESI) Mass Spectrometry

    Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
    ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
    Peptide Identification Using Tandem Mass Spectrometry01:33

    Peptide Identification Using Tandem Mass Spectrometry

    Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
    This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
    Mass Spectrometry: Complex Analysis01:21

    Mass Spectrometry: Complex Analysis

    Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
    GC–MS is a powerful hyphenated method commonly used in forensics and environmental...