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Updated: Jun 6, 2026

Measuring Enzymatic Stability by Isothermal Titration Calorimetry
Published on: March 26, 2019
Simultaneous determination of equilibrium constants and enthalpy changes by titration calorimetry: Methods,
Lee D Hansen1, Gilbert W Fellingham, Donald J Russell
1Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA. lee_hansen@byu.edu
Abstract:
Calorimetric methods have been used to determine equilibrium constants since 1937, but no comprehensive review of the various calorimeters and methods has been done previously. This article reports methods for quantitative comparison of the capabilities of calorimeters for simultaneous determination of equilibrium constants and enthalpy changes, for determining optimal experimental conditions, and for assessing the effects of systematic and random errors on the accuracy and precision of equilibrium constants and enthalpy changes determined by this method.
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