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Grade III Varicocele Surgical Treatment using Spermatic Vein-Superficial Abdominal Vein Shunt

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Adolescent varicocele: improved sperm function after varicocelectomy.

Jose Iran Lacerda¹, Paula Toni Del Giudice, Barbara Ferreira da Silva

¹Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University, Sao Paulo, Brazil.

Fertility and Sterility

|November 16, 2010

Summary

Varicocelectomy in adolescents improves sperm DNA integrity and mitochondrial activity. However, it does not affect seminal lipid peroxidation levels.

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Area of Science:

Reproductive Medicine
Andrology
Spermatology

Background:

Varicocele is a common cause of male infertility.
Adolescent varicocele can negatively impact sperm function.
Assessing varicocelectomy's effect on sperm quality in adolescents is crucial.

Purpose of the Study:

To evaluate the impact of varicocelectomy on sperm DNA integrity and mitochondrial activity in adolescents.
To assess changes in seminal lipid peroxidation after varicocelectomy in this age group.

Main Methods:

Prospective study involving adolescents (14-19 years) with varicocele (grades II-III).
Semen analysis performed before and three months after subinguinal microsurgical varicocelectomy.
Sperm DNA fragmentation (Comet assay), mitochondrial activity (diaminobenzidine assay), and malondialdehyde levels were measured.

Main Results:

Varicocelectomy significantly increased sperm with intact DNA (grade I) and reduced DNA fragmentation (grades II-IV).
Sperm mitochondrial activity improved, with fewer cells showing inactive mitochondria (class III).
No significant difference was observed in seminal lipid peroxidation (malondialdehyde) levels.

Conclusions:

Adolescent varicocelectomy enhances sperm DNA integrity and mitochondrial function.
The procedure does not alter seminal lipid peroxidation markers.
Varicocelectomy is a viable option for improving sperm quality in adolescents with varicocele.