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The effects of Sp7/Osterix gene silencing in the chondroprogenitor cell line, ATDC5

Kazuki Omoteyama1, Minoru Takagi

  • 1Department of Anatomy, Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, 1-8-13, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan. omoteyama@dent.nihon-u.ac.jp

Biochemical and Biophysical Research Communications
|November 16, 2010
PubMed

Insights

Sp7/Osterix (Osx) is crucial for chondrocyte differentiation, regulating key genes and alkaline phosphatase activity. Bone morphogenetic protein 2 (BMP2) enhances Osx expression, promoting chondrogenesis.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Chondrocytes express Sp7/Osterix (Osx), but its specific role in chondrocytes remains unclear.
  • The ATDC5 cell line, a clonal mouse embryonic cell line, retains chondroprogenitor properties and expresses Osx.

Purpose of the Study:

  • To investigate the function of the Osx gene in chondrocytes.
  • To determine the effect of Osx gene silencing on chondrocyte marker gene expression and alkaline phosphatase (ALP) activity.

Main Methods:

  • Utilized shRNA lentiviral particles for Osx gene silencing in ATDC5 cells.
  • Assessed chondrocyte marker gene expression (Sox trio, Dlx5, Alp, Col X) and ALP enzyme activity.
  • Investigated the impact of Bone morphogenetic protein 2 (BMP2) stimulation on Osx expression and chondrogenesis.

Main Results:

  • Osx gene silencing down-regulated Sox trio, Dlx5, and Alp mRNA expression, and reduced ALP activity.
  • BMP2 stimulation rescued Osx expression and dose-dependently increased Alp expression and ALP activity.
  • Osx silencing inhibited hypertrophic chondrocyte marker gene (Col X) expression and attenuated Dlx5 and Alp mRNA upregulation.

Conclusions:

  • Osx plays a significant role in activating chondrogenic genes.
  • Osx acts as a positive regulator in the process of chondrocyte differentiation.

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