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Related Experiment Video

Updated: Jun 6, 2026

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

Published on: March 15, 2021

Three-dimensional cellular ultrastructure resolved by X-ray microscopy.

Gerd Schneider¹, Peter Guttmann, Stefan Heim

¹Helmholtz Zentrum Berlin für Materialien und Energie GmbH, Wilhelm-Conrad-Röntgen Campus, Berlin, Germany.

|November 16, 2010

Summary

This summary is machine-generated.

Researchers created a new X-ray microscope for imaging frozen cells. This advanced technology achieved high resolution, revealing detailed cellular structures like nuclear membranes and mitochondria in 3D.

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Visualization of Organelles In Situ by Cryo-STEM Tomography

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

Area of Science:

Cell Biology
Microscopy
Biophysics

Background:

Conventional X-ray microscopy often uses quasi-incoherent illumination.
Imaging intact, frozen-hydrated cells at high resolution presents significant challenges.

Purpose of the Study:

To develop an advanced X-ray microscope for high-resolution 3D imaging of intact, frozen-hydrated cells.
To enable visualization of subcellular structures in adherent cells.

Main Methods:

Utilized partially coherent object illumination in an X-ray microscope design.
Integrated a cryogenic tilt stage for tomography of frozen-hydrated samples.
Performed 3D reconstruction of mouse adenocarcinoma cells.

Main Results:

Achieved approximately 36-nm (Rayleigh) and 70-nm (Fourier ring correlation) resolution.
Successfully visualized the double nuclear membrane and nuclear pores.
Identified mitochondrial cristae and lysosomal inclusions within cells.

Conclusions:

The developed X-ray microscope enables high-resolution 3D imaging of cellular ultrastructure.
Partially coherent illumination is effective for advanced cryo-tomography.
This technique provides new insights into the morphology of intact, adherent cells.