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Updated: Jun 6, 2026
Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
Published on: August 24, 2015
Evaluation of Different Preparation Procedures of Pathogen Reduction Technology(Mirasol®)-Treated Platelets Collected
Karin Janetzko1, Katharina Hinz, Susanne Marschner
1Institute of Transfusion Medicine and Immunology, Red Cross Service of Baden-Württemberg - Hessen, Medical Faculty of Mannheim, Heidelberg University, Mannheim, Germany.
Abstract:
SUMMARY: BACKGROUND: The Mirasol® pathogen reduction technology (PRT) for platelet concentrates (PC) uses riboflavin and UV light (270-360 nm). We evaluated the impact of PRT on platelets in comparison to standard single-donor PC. MATERIAL AND METHODS: Platelets were resuspended in autologous plasma. After 2 h rest without agitation, PC were split into an untreated control unit (C-PC) and an immediately treated unit (T-PC) (series I). In series IV, split PC were stored under agitation over night before PRT was carried out. Platelet quality was assessed by pH, glucose consumption, lactate production rate, LDH, soluble sCD62p and CD62p expression with and without TRAP (thrombin receptor-activating peptide) over 7 days. RESULTS: SERIES I: On day 5, pH values were lower for T-PC (6.8 ± 0.2 vs. 7.4 ± 0.1, C-PC), accompanied by a higher glucose consumption rate of 0.069 ± 0.016 vs. 0.035 ± 0.006 mmol/10(12) platelets/h and lactate production rate of 0.126 ± 0.031 vs. 0.063 ± 0.011 mmol/10(12) platelets/h. CD62p using TRAP was lower for T-PC (50 ± 11 vs. 62 ± 14%). Baseline activation was higher in T-PC (35 ± 12 vs. 28 ± 15%). Longer initial rest time had no impact on these results (series II/III/IV). CONCLUSION: PRT leads to an increase of platelet metabolism and activation independent of the length of the initial rest times. PC resuspended in autologous plasma should be stored at maximum up to day 5.
Insights
The Mirasol® pathogen reduction technology (PRT) increases platelet metabolism and activation. Platelet concentrates treated with PRT should be stored for a maximum of 5 days.
Area of Science:
- Hematology
- Transfusion Medicine
- Biotechnology
Background:
- Mirasol® pathogen reduction technology (PRT) utilizes riboflavin and UV light for pathogen inactivation in platelet concentrates (PC).
- Understanding the impact of PRT on platelet quality and function is crucial for transfusion safety and efficacy.
Purpose of the Study:
- To evaluate the effects of Mirasol® PRT on platelet quality parameters compared to standard single-donor PC.
- To assess the influence of PRT on platelet metabolism, activation markers, and storage viability.
Main Methods:
- Platelet concentrates (PC) were processed with Mirasol® PRT (treated, T-PC) or left untreated (control, C-PC).
- Platelet quality was assessed over 7 days, measuring pH, glucose consumption, lactate production, LDH, and CD62p expression.
- Experiments included immediate treatment and treatment after overnight storage under agitation.
Main Results:
- Treated PC exhibited lower pH and increased glucose consumption and lactate production rates by day 5.
- CD62p expression (a marker of platelet activation) was altered in T-PC, with higher baseline activation.
- Initial rest time before PRT did not significantly alter these observed effects.
Conclusions:
- Mirasol® PRT enhances platelet metabolism and activation, irrespective of initial rest duration.
- Platelet concentrates treated with PRT and resuspended in autologous plasma have a recommended maximum storage duration of 5 days.
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