[Freezing of dehydrated calluses of spring wheat (Triticum aestivum L.) in liquid nitrogen and their morphogenetic...

A cryopreservation method developed earlier was modified for freezing the calluses derived from mature embryos of four spring wheat (Triticum aestivum L.) pure lines. The effects of individual stages of cryopreservation protocol on the water content, number of calluses resuming growth, and rate of morphogenesis were analyzed. Growth resumption was observed in 90.5-100% of the calluses after dehydration and in 93.3-100% after freezing-thawing. The number of calluses displaying signs of morphogenesis considerably decreased after dehydration but remained almost the same after freezing-thawing.