Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Colloids03:22

Colloids

Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
The Colloidal State01:29

The Colloidal State

The formation of a colloidal system is exemplified by an aqueous solution containing Cl− ions is introduced to another containing Ag+ ions, resulting in the precipitation of solid AgCl as extremely tiny crystals. Instead of settling out as a filterable precipitate, these crystals remain suspended in the liquid, showcasing a colloidal system.A colloidal system involves colloidal particles within the approximate range of 1 to 1000 nm in at least one dimension, dispersed in a medium called the...
Colloidal precipitates01:09

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
Colloids and Suspensions01:17

Colloids and Suspensions

Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles visible to the naked eye or seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. The suspended particles in a suspension settle out after some time of mixing. The separation of particles from a suspension is...
Qualitative Analysis03:46

Qualitative Analysis

For solutions containing mixtures of different cations, the identity of each cation can be determined by qualitative analysis. This technique involves a series of selective precipitations with different chemical reagents, each reaction producing a characteristic precipitate for a specific group of cations. Metal ions within a group are further separated by varying the pH, heating the mixture to redissolve a precipitate, or adding other reagents to form complex ions.
For instance, group IV...
Coagulation01:06

Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transparency-changing elastomers by controlling of the refractive index of liquid inclusions.

Journal of physics. Condensed matter : an Institute of Physics journal·2024
Same author

Ferrous Pyrophosphate and Mixed Divalent Pyrophosphates as Delivery Systems for Essential Minerals.

ACS food science & technology·2024
Same author

Thermoresponsive oil-continuous gels based on double-interpenetrating colloidal-particle networks.

Soft matter·2024
Same author

Pickering stabilization mechanism revealed through direct imaging of particles with tuneable contact angle in a phase-separated binary solvent.

Journal of colloid and interface science·2024
Same author

Interactions of Natural Flavones with Iron Are Affected by 7-<i>O</i>-Glycosylation, but Not by Additional 6″-<i>O-</i>Acylation.

ACS food science & technology·2023
Same author

Is there a difference between surfactant-stabilised and Pickering emulsions?

Soft matter·2023
Same journal

Discovery of Novel Pyrazole-Benzothiazine Hybrids as Potential HPPD Inhibitors.

Journal of agricultural and food chemistry·2026
Same journal

Structural Regulation of Lignin Using an Al<sup>3+</sup>/Zn<sup>2+</sup> Bimetallic Lewis Acid System to Enhance Enzymatic Saccharification.

Journal of agricultural and food chemistry·2026
Same journal

Comparative NMR-Based Metabolomic and Functional Assessment of Fruit and Vegetable Extracts under Regenerative Agricultural Practices.

Journal of agricultural and food chemistry·2026
Same journal

Fenoxaprop-<i>P</i>-ethyl Disrupts Lipid Homeostasis in Rice Seedlings: A Multiomics Study Linking the Inhibition of Acetyl-Coenzyme A Carboxylase with a Coordinated Repression of Fatty Acid Elongation Processes.

Journal of agricultural and food chemistry·2026
Same journal

Exploiting Biomacromolecular Oligomerization for Next-Generation Pesticide Discovery: Challenges and Perspectives.

Journal of agricultural and food chemistry·2026
Same journal

Design, Synthesis, and Insecticidal Potential of 1,2,4-Oxadiazole-Fused Isoxazoline Derivatives as GABA Receptor Antagonists.

Journal of agricultural and food chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

Zinc-Sponge Battery Electrodes that Suppress Dendrites
06:58

Zinc-Sponge Battery Electrodes that Suppress Dendrites

Published on: September 29, 2020

Sodium caseinate stabilized zein colloidal particles.

Ashok R Patel1, Elisabeth C M Bouwens, Krassimir P Velikov

  • 1Unilever R&D Vlaardingen, Olivier van Noortlaan 120, 3133 AC Vlaardingen, The Netherlands.

Journal of Agricultural and Food Chemistry
|November 17, 2010
PubMed
Summary
This summary is machine-generated.

Researchers stabilized zein colloidal particles using sodium caseinate, creating stable, redispersible nanoparticles. These natural particles show potential for delivering bioactive molecules in various formulations.

More Related Videos

A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating
09:01

A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating

Published on: July 8, 2016

Related Experiment Videos

Last Updated: Jun 6, 2026

Zinc-Sponge Battery Electrodes that Suppress Dendrites
06:58

Zinc-Sponge Battery Electrodes that Suppress Dendrites

Published on: September 29, 2020

A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating
09:01

A Protocol for the Production of Gliadin-cyanoacrylate Nanoparticles for Hydrophilic Coating

Published on: July 8, 2016

Area of Science:

  • Food Science and Technology
  • Materials Science
  • Biotechnology

Background:

  • Zein, a prolamin protein, is a potential biopolymer for encapsulation but prone to aggregation near its isoelectric point.
  • Developing stable colloidal systems from natural proteins is crucial for food and pharmaceutical applications.
  • Sodium caseinate offers electrosteric stabilization properties for protein-based nanoparticles.

Purpose of the Study:

  • To prepare and stabilize zein colloidal particles using sodium caseinate.
  • To characterize the size, surface potential, and stability of the stabilized particles.
  • To evaluate the potential of these particles as delivery systems for bioactive molecules.

Main Methods:

  • Antisolvent precipitation method for zein colloidal particle preparation.
  • Sodium caseinate used as an electrosteric stabilizer.
  • Particle characterization including size, surface potential, isoelectric point (IEP) determination, and stability studies (ionic strength, drying).
  • In vitro protein hydrolysis assay to assess digestibility.

Main Results:

  • Well-defined zein colloidal particles (120-150 nm) with negative surface potential (-29 to -47 mV) were successfully prepared.
  • Sodium caseinate shifted the zein isoelectric point from pH 6.0 to pH 5.0, preventing aggregation near native pH (6.2).
  • Stabilized particles exhibited excellent stability across a wide range of ionic strengths (15 mM-1.5 M NaCl) and retained redispersibility after drying.
  • In vitro digestibility studies confirmed that caseinate stabilization did not compromise zein's inherent digestibility.

Conclusions:

  • Sodium caseinate effectively stabilizes zein colloidal particles against aggregation and environmental stresses.
  • The stabilized zein-caseinate colloidal particles are redispersible and maintain protein digestibility.
  • These all-natural colloidal particles represent a promising platform for encapsulating and delivering bioactive compounds in food, pharmaceutical, and agricultural sectors.