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Updated: Jun 6, 2026

Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis

Pupal and Adult Injections for RNAi and CRISPR Gene Editing in Nasonia vitripennis

Published on: December 4, 2020

Citrus, a key insect eggshell protein.

Paula Irles¹, Maria-Dolors Piulachs

¹Institut de Biologia Evolutiva (CSIC-UPF), Passeig Marítim de Barceloneta, 37, 08003 Barcelona, Spain. pirles@gmail.com

Insect Biochemistry and Molecular Biology

|November 17, 2010

Summary

Researchers identified a new gene, Citrus, crucial for insect eggshell (chorion) formation in cockroaches. This discovery enhances our understanding of chorion synthesis in insects with panoistic ovaries.

Area of Science:

Insect reproductive biology
Molecular genetics
Biochemistry

Background:

Chorion synthesis molecular data is limited in insects with panoistic ovaries.
Previous work identified novel genes in Blattella germanica chorion formation.

Purpose of the Study:

To characterize the novel chorion gene, Citrus, from Blattella germanica.
To elucidate the function of Citrus in endochorion development.

Main Methods:

Gene identification and characterization.
RNA interference (RNAi) techniques to assess gene function.

Main Results:

A novel gene, Citrus, was identified in Blattella germanica.
Citrus gene product features a highly repetitive motif.

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RNAi experiments confirmed Citrus's essential role in endochorion formation.

Conclusions:

Citrus is a key gene in the molecular mechanism of endochorion synthesis in Blattella germanica.
This study contributes to understanding insect eggshell development in species with panoistic ovaries.