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Updated: Jun 6, 2026
Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
Published on: September 21, 2019
Reducing acyl migration during purification of 2-arachidonoylglycerol from biological samples before gas
Sei Higuchi1, Keiichi Irie, Takafumi Nakano
1Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Fukuoka 814–0180, Japan.
Abstract:
Endocannabinoid 2-arachidonoylglycerol (2-AG) regulates several important physiological processes in the brain. 2-AG is commonly quantified by gas chromatography mass spectrometry after an initial purification step. The most precise and rapid purification utilizes C(18) solid-phase extraction, but quantification problems can arise with acyl migration from 2-AG to 1-arachidonoylglycerol. We found that extraction with methanol promoted this migration, but acetone and diethyl ether (Et(2)O) did not. Acetone and Et(2)O were used to develop a purification method for the direct determination of 2-AG.
