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Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
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Inducible transgenic mouse models.

Thomas L Saunders1

  • 1Transgenic Animal Model Core, University of Michigan, Ann Arbor, MI, USA. tsaunder@umich.edu

Methods in Molecular Biology (Clifton, N.J.)
|November 17, 2010
PubMed
Summary
This summary is machine-generated.

Inducible transgenic mouse models offer precise control over gene expression. Tetracycline-regulated and estrogen receptor (ER)-based systems enable cell-specific gene activation or inactivation at specific times.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • Inducible transgenic mouse models are crucial for studying gene function in specific cells and tissues.
  • Effective models require low background gene expression and high inducibility with minimal agent dosage.
  • Common control systems include tetracycline-operon and estrogen receptor (ER) ligand-binding domains.

Purpose of the Study:

  • To review and compare methods for inducible gene expression in transgenic mouse models.
  • To highlight the utility of tetracycline-regulated and ER-based systems for temporal and spatial gene control.
  • To discuss the complementary roles of these systems in gene activation and inactivation.

Main Methods:

  • Overview of established inducible gene expression systems in mice.
  • Focus on tetracycline-regulated bi-transgenic systems.
  • Discussion of estrogen receptor (ER) ligand-binding domain fusions, particularly with Cre recombinase (Cre-ER).
  • Mention of less common systems like progesterone receptor, lac, and GAL4.

Main Results:

  • Tetracycline-regulated models allow temporal and cell-specific gene activation.
  • Cre-ER systems enable inducible recombinase activity for gene manipulation.
  • Cre recombinase is frequently used with conditional alleles for gene inactivation.
  • Cre can also activate gene expression by removing stop cassettes.

Conclusions:

  • Tetracycline-regulated and Cre-ER systems are complementary tools in transgenic mouse research.
  • These systems provide precise control over gene activation and inactivation.
  • They are valuable for studying gene function in a cell-specific and time-dependent manner.