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Updated: Jun 6, 2026

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
10:21

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Lentiviral transgenesis.

Terunaga Nakagawa1, Casper C Hoogenraad

  • 1Department of Chemistry and Biochemistry, University of California, San Diego, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 17, 2010
PubMed
Summary
This summary is machine-generated.

Lentiviral vectors offer an efficient alternative to traditional DNA injection for creating transgenic mice and rats. This guide details methods for lentiviral vector generation and application, facilitating researcher transition.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Animal Models

Background:

  • Conventional DNA injection for transgenic animal generation has seen limited innovation.
  • Lentiviral vectors provide a highly efficient method for genomic DNA integration.
  • This chapter focuses on lentiviral vector applications in generating transgenic rodents.

Purpose of the Study:

  • To provide a detailed protocol for generating transgenic mice and rats using lentiviral vectors.
  • To enable researchers familiar with pronuclear injection to adopt lentiviral transgenesis.
  • To cover all aspects from vector production to troubleshooting.

Main Methods:

  • Generation of lentiviral vectors and production of high-titer lentiviral particles.
  • Microinjection of lentivirus into fertilized oocytes.
  • Embryo transfer into pseudopregnant females.
  • Detailed surgical and laboratory protocols.

Main Results:

  • Successful generation of transgenic mice and rats using lentiviral vectors.
  • Demonstration of efficient genomic DNA incorporation.
  • Establishment of a comprehensive protocol for lentiviral transgenesis.

Conclusions:

  • Lentiviral transgenesis is a powerful and efficient alternative for generating transgenic rodents.
  • The provided methods facilitate the adoption of lentiviral techniques by researchers.
  • Understanding limitations and troubleshooting are key for successful implementation.