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Generation of Genetically Modified Mice through the Microinjection of Oocytes
Published on: June 15, 2017
Transgenesis in mouse embryonic stem cells
Janine van Ree1, Wei Zhou, Ming Li
1Department of Pediatrics and Adolescent Medicine, Mayo Clinic College of Medicine, Rochester, MN, USA. vanree.janine@mayo.edu
Methods in Molecular Biology (Clifton, N.J.)
|November 17, 2010
Summary
This study presents new methods for creating transgenic mice using embryonic stem (ES) cells. These protocols offer improved control over gene expression and overcome limitations of traditional transgenic mouse generation.
Area of Science:
- Genetics and Genomics
- Molecular Biology
- Animal Models
Background:
- Traditional transgenic mouse generation relies on pronuclear injection of DNA.
- This method faces challenges like poor transgene expression control, cytotoxicity, and founder sterility.
Purpose of the Study:
- To develop novel protocols for generating transgenic mice.
- To enable inducible transgene expression and overcome limitations of existing methods.
Main Methods:
- Utilizing embryonic stem (ES) cell clones with stably integrated exogenous DNA.
- Implementing two distinct protocols for generating transgenic mice from these ES cells.
Main Results:
- Successfully generated transgenic mice using the described ES cell-based protocols.
- Achieved inducible control over transgene expression in the generated mice.
Conclusions:
- The developed ES cell-based protocols offer a viable alternative for transgenic mouse generation.
- These methods provide enhanced control over transgene expression and mitigate issues associated with traditional techniques.
