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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: Jun 6, 2026

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

Generating conditional knockout mice.

Roland H Friedel¹, Wolfgang Wurst, Benedikt Wefers

¹Mount Sinai School of Medicine, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 17, 2010

Summary

This summary is machine-generated.

Conditional gene inactivation using Cre-lox technology allows researchers to study gene function in specific cell types and adult mice. This method enables precise control over gene knockout, overcoming limitations of traditional methods.

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Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Area of Science:

Genetics
Molecular Biology
Developmental Biology

Background:

Gene targeting in embryonic stem (ES) cells is crucial for creating mouse models to study gene function.
Traditional knockout mice often result in embryonic lethality, limiting studies in adult or specific cell types.
Conditional gene inactivation strategies are needed to overcome these limitations.

Purpose of the Study:

To describe a refined strategy for conditional gene inactivation using Cre-lox technology.
To detail the design and construction of loxP-flanked alleles.
To outline the use of Cre and CreER(T2) transgenic mice for inducible gene inactivation.

Main Methods:

Utilizing Cre-lox recombination to excise floxed gene segments.
Generating conditional mutant mice by crossing floxed strains with Cre transgenic lines.
Employing CreER(T2) transgenic mice for tamoxifen-inducible gene inactivation in adult mice.
Leveraging vectors, ES cells, and mice from the EUCOMM project.

Main Results:

Development of a versatile system for conditional gene inactivation in mice.
Establishment of numerous Cre transgenic lines for cell-type-specific gene targeting.
Availability of inducible systems (CreER(T2)) for temporal control of gene inactivation.
Facilitation of phenotype analysis in conditional mutants.

Conclusions:

Conditional gene inactivation via Cre-lox technology is a powerful tool for studying gene function in vivo.
This strategy enables precise temporal and spatial control over gene knockout.
The EUCOMM project provides essential resources for generating and analyzing conditional mouse models.