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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 6, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

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Published on: April 2, 2014

MICER targeting vectors for manipulating the mouse genome.

Chunhong Liu1, Paul F Szurek, Y Eugene Yu

  • 1Genetics Program and Department of Cancer Genetics, Roswell Park Cancer Institute, New York State Center of Excellence in Bioinformatics and Life Sciences Buffalo, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 17, 2010
PubMed
Summary

Mice are crucial models for human biology research. A new resource now provides essential tools, speeding up the creation of genetically modified mice for disease studies.

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Last Updated: Jun 6, 2026

Mouse Genome Engineering Using Designer Nucleases
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10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Area of Science:

  • * Genetics and Genomics
  • * Mammalian Model Organisms
  • * Molecular Biology

Background:

  • * Mice are vital models for studying human development, physiology, and diseases due to genetic and biological similarities.
  • * Gene targeting in mouse embryonic stem cells allows for precise genetic modifications.
  • * Constructing targeting vectors is a time-consuming bottleneck in gene-targeting experiments.

Purpose of the Study:

  • * To introduce the Mutagenic Insertion and Chromosome Engineering Resource.
  • * To highlight the availability of targeting vectors for insertional mutagenesis and chromosome engineering.
  • * To emphasize the resource's role in advancing targeted manipulation of the mouse genome.

Main Methods:

  • * Development and provision of targeting vectors for insertional mutagenesis.
  • * Creation of tools for chromosome engineering across the mouse genome.
  • * Establishment of a centralized resource for genetic reagents.

Main Results:

  • * The Mutagenic Insertion and Chromosome Engineering Resource offers a wide array of targeting vectors.
  • * These vectors facilitate insertional mutagenesis for numerous mouse genes.
  • * The resource enables comprehensive chromosome engineering throughout the mouse genome.

Conclusions:

  • * The new resource significantly accelerates the generation of desired mouse mutants.
  • * It enriches the available genetic reagents for targeted mouse genome manipulation.
  • * This advancement streamlines research in mouse models for human biology and disease.