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Related Concept Videos

Persuasion Strategies01:52

Persuasion Strategies

Researchers have tested many persuasion strategies, including the foot-in-the door and the door-in-the-face techniques, in a variety of contexts. Ultimately, the principles are effective in selling products and changing people’s attitude, ideas, and behaviors (Cialdini & Goldstein, 2004).
Impression Management Techniques II: Ingratiation01:29

Impression Management Techniques II: Ingratiation

Ingratiation refers to deliberate behaviors aimed at increasing one’s attractiveness or likability to a target person, often for strategic interpersonal or social gain. This set of impression management tactics is especially prevalent in hierarchical contexts, where influencing someone with greater power or authority can yield significant benefits. Several distinct ingratiation strategies have been identified, each leveraging psychological cues to foster favor and affiliation.Opinion...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Impression Management Techniques III: Aligning Actions01:29

Impression Management Techniques III: Aligning Actions

Aligning actions are communicative strategies individuals employ to maintain social harmony and preserve personal identity in the face of potential disruptions to social norms. These actions are particularly important in managing social impressions when one's behavior might be seen as inappropriate, incompetent, or morally questionable.Types of Aligning ActionsThe three principal types of aligning actions are disclaimers, accounts, and apologies.DisclaimersDisclaimers are preventive; they are...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
11:32

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines

Published on: November 13, 2021

Knock-in approaches.

Anton J M Roebroek1, Philip L S M Gordts, Sara Reekmans

  • 1Laboratory for Experimental Mouse Genetics, Center for Human Genetics, K.U. Leuven, Leuven, Belgium. anton.roebroek@med.kuleuven.be

Methods in Molecular Biology (Clifton, N.J.)
|November 17, 2010
PubMed
Summary
This summary is machine-generated.

New knock-in strategies for mouse models are advancing gene function studies and disease modeling. Recombinase-mediated cassette exchange offers an efficient method for generating diverse knock-in mutations in mice.

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Area of Science:

  • Molecular genetics
  • Gene function studies
  • Mouse models for human diseases

Background:

  • Developing advanced molecular genetic strategies is crucial for understanding gene function.
  • Mouse models are essential tools for studying human diseases.
  • Knock-in approaches are increasingly important for genetic research.

Purpose of the Study:

  • To elaborate on novel developments in generating knock-in mice.
  • To highlight the significance of emerging knock-in strategies.
  • To focus on recombinase-mediated cassette exchange for generating knock-in mutations.

Main Methods:

  • Review of novel developments in knock-in mouse generation.
  • Emphasis on recombinase-mediated cassette exchange (RMCE).
  • Application of RMCE for creating multiple knock-in mutations.

Main Results:

  • Advancements in generating knock-in mice are presented.
  • Recombinase-mediated cassette exchange is identified as a key emerging strategy.
  • RMCE facilitates the easy generation of a series of knock-in mutations within a single gene.

Conclusions:

  • Novel knock-in strategies are continuously being developed.
  • Recombinase-mediated cassette exchange represents a significant advancement in generating knock-in mice.
  • This method simplifies the creation of diverse genetic modifications for research.