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Updated: Jun 6, 2026
05:56
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Published on: November 12, 2020
Proteomics and the dynamic plasma membrane: Quo Vadis?
Richard R Sprenger1, Ole N Jensen
1Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
Proteomics
|November 17, 2010
Summary
Mass spectrometry (MS)-based proteomics now tracks dynamic changes in the plasma membrane proteome. This advancement is key for understanding cellular functions, diseases, and identifying new drug targets.
Area of Science:
- Proteomics
- Cell Biology
- Biochemistry
Background:
- The plasma membrane acts as a crucial cellular barrier and signaling hub.
- Understanding its dynamic proteome is vital for biological and disease research.
- Current proteomics methods are evolving to capture membrane protein dynamics.
Purpose of the Study:
- To review recent advancements in mass spectrometry (MS)-based plasma membrane proteomics.
- To highlight the challenges and importance of studying the dynamic plasma membrane proteome.
- To showcase progress in eukaryotic systems, including mammals, yeast, and plants.
Main Methods:
- Mass spectrometry (MS)-based proteomics techniques.
- Enrichment strategies for plasma membrane proteins.
- Quantitative proteomics for analyzing dynamic changes.
- Post-translational modification (PTM) analysis.
Main Results:
- MS-based proteomics enables monitoring of plasma membrane protein abundance, location, and PTMs.
- Key examples from mammals, yeast, and plants demonstrate recent progress.
- Technological advancements are crucial for detailed functional and comparative proteomic analyses.
Conclusions:
- The dynamic plasma membrane proteome is increasingly accessible to MS-based analysis.
- Characterizing these dynamics is essential for understanding cellular processes and disease.
- Further development in enrichment and quantification technologies will drive future discoveries.
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