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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
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Reference spectra from squamous epithelium and connective tissue allow whole section proteomics analysis.

Mariana Roesch-Ely1, Martina Schnölzer, Matthias Nees

  • 1Molecular Biology Laboratory, Department of Otolaryngology, Head and Neck Surgery, University of Heidelberg, Heidelberg, Germany.

Archives of Physiology and Biochemistry
|November 18, 2010
PubMed
Summary

Omitting micro-dissection in cancer studies reveals crucial early interactions between tumor cells and their environment. This approach highlights key protein changes in head and neck squamous cell carcinoma development.

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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Area of Science:

  • Oncology
  • Proteomics
  • Biochemistry

Background:

  • Tumor-stroma interactions are critical in solid tumor biology.
  • Micro-dissection of tumor cells may omit essential microenvironmental context.
  • Understanding early changes in head and neck squamous cell carcinoma (HNSCC) requires studying these interactions.

Purpose of the Study:

  • To investigate the role of tumor microenvironment interactions in HNSCC development.
  • To identify specific proteins involved in early stages of HNSCC.
  • To evaluate the benefit of omitting micro-dissection in proteomic studies of solid tumors.

Main Methods:

  • Generated reference protein spectra for normal squamous epithelium and connective tissue using Surface-Enhanced Laser Desorption/Ionization-Time Of Flight Mass Spectrometry (SELDI-TOF-MS).
  • Analyzed protein expression profiles in normal tissues and early pre-malignant lesions of HNSCC.

Main Results:

  • Identified epithelial proteins: Calgranulins A and B, Annexin 1, and Histone H4.
  • Identified connective tissue proteins: alpha-defensins 1-3 and hemoglobin subunits.
  • Observed up-regulation of stromal alpha-defensins and down-regulation of epithelial Annexin 1 in tumor-distant epithelia (early lesions).

Conclusions:

  • Tumor microenvironment interactions are involved very early in the carcinogenic process.
  • Omitting micro-dissection is beneficial for studying protein expression changes during solid tumor development and progression.
  • These findings provide insights into the early molecular events driving HNSCC.