Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct Lineages of <i>Pantoea ananatis</i> Associated with Rice Differ in Toxin Biosynthesis Gene Content and Host Specialization.

Phytopathology·2026
Same author

Functional and Regulatory Complexity: Challenges and Prospects for Understanding Rice WRKY Transcription Factors.

Journal of experimental botany·2026
Same author

The conserved nematode pheromone ascr#18 primes plant immunity.

Communications biology·2026
Same author

Shortened High-dose Palliative Radiotherapy for Lung Cancer (SHiP-Rt): protocol for a single-arm, multicentre, phase II study.

BMJ open·2026
Same author

Unraveling the Dynamics of <i>Pantoea ananatis</i>: Isolation and Plant Responses.

Phytopathology·2026
Same author

Crop growth model-enabled genetic mapping of biomass accumulation dynamics in photoperiod-sensitive sorghum.

The plant genome·2025

Related Experiment Video

Updated: Jun 6, 2026

Metagenomic Analysis of Silage
08:43

Metagenomic Analysis of Silage

Published on: January 13, 2017

Crop genome sequencing: lessons and rationales.

Catherine Feuillet1, Jan E Leach, Jane Rogers

  • 1Institut National de la Recherche Agronomique-Université Blaise Pascal-UMR1095-Domaine de Crouel, 63100 Clermont-Ferrand, France. catherine.feuillet@clermont.inra.fr

Trends in Plant Science
|November 18, 2010
PubMed
Summary

The 10th anniversary of the first plant genome sequence highlights advancements in crop sequencing. However, the focus on draft genomes, unlike the high-quality rice genome, may hinder evolutionary and phenotypic research.

More Related Videos

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

Related Experiment Videos

Last Updated: Jun 6, 2026

Metagenomic Analysis of Silage
08:43

Metagenomic Analysis of Silage

Published on: January 13, 2017

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
10:19

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

Area of Science:

  • Plant genomics
  • Genomic sequencing technologies
  • Evolutionary biology

Background:

  • The first plant genome sequence (Arabidopsis thaliana) was completed in 2000.
  • Advancements in sequencing technologies have led to numerous crop genome projects.
  • Eight crop genome sequences have been published since 2008.

Purpose of the Study:

  • To review current crop genome sequencing activities.
  • To discuss the impact of sequence quality on research utility.
  • To propose a future direction for crop genome sequencing priorities.

Main Methods:

  • Literature review of published crop genome sequences.
  • Analysis of sequence quality metrics.
  • Discussion of research applications affected by genome sequence quality.

Main Results:

  • While many crop genomes have been sequenced, only rice (Oryza sativa) has achieved a quality comparable to Arabidopsis thaliana.
  • The prevalence of draft genomes may limit detailed studies on speciation, evolution, and genotype-phenotype correlations.
  • Variability in sequence quality significantly affects the usability of genomic data for different research questions.

Conclusions:

  • A shift in strategy is needed to prioritize high-quality genome sequencing for key crops.
  • Future sequencing efforts should focus on maximizing the utility of genomic data for diverse biological inquiries.
  • Ensuring high-quality genome assemblies is crucial for advancing plant science and crop improvement.