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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Updated: Jun 6, 2026

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

A plasmid-based multigene expression system for mammalian cells.

Andrijana Kriz¹, Katharina Schmid, Nadia Baumgartner

¹Paul Scherrer Institute, Biomolecular Research, Molecular Cell Biology, Villigen CH-5232, Switzerland.

Nature Communications

|November 18, 2010

Summary

This summary is machine-generated.

Introducing MultiLabel, a novel system for efficient multigene expression in mammalian cells. This modular plasmid-based approach simplifies the creation of genetically modified cells for research.

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Area of Science:

Molecular Biology
Cell Biology
Biotechnology

Background:

Introducing multiple genes into mammalian cells is crucial for analyzing cellular processes and manipulating cell fate.
Existing methods for multigene expression are often inefficient, inflexible, and time-consuming.
There is a need for improved technologies to streamline the process of eukaryotic gene expression.

Purpose of the Study:

To develop a novel, efficient, and modular plasmid-based eukaryotic expression system for multigene expression in mammalian cells.
To overcome the limitations of current technologies in terms of efficiency, flexibility, and time.

Main Methods:

Development of the MultiLabel system, a modular plasmid-based eukaryotic expression vector.
Assembly of independent expression vectors into a multi-gene plasmid using a Cre/LoxP recombination system.
Application of the system for the rapid construction of multigene expression vectors.

Main Results:

The MultiLabel system enables highly efficient multigene expression in mammalian cells.
Rapid construction of multigene expression vectors is achieved through modular assembly.
Facilitates single-step creation of transiently or stably transfected mammalian cells.

Conclusions:

MultiLabel represents a significant advancement in eukaryotic expression systems for multigene delivery.
The system offers a rapid, efficient, and flexible solution for researchers working with mammalian cell genetics.
Enables streamlined experimental workflows in molecular and cell biology research.