Related Experiment Video
Updated: Jun 6, 2026
10:50
Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
Published on: April 7, 2023
Isolation of murine adipose-derived stem cells
Gang Yu1, Xiying Wu, Gail Kilroy
1Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 18, 2010
Summary
This study details methods for isolating, expanding, and storing mouse adipose-derived stem cells. These cells can be differentiated into adipocytes to study metabolic disorders.
Area of Science:
- Biochemistry
- Cell Biology
- Metabolic Research
Background:
- Murine models are crucial for studying obesity and adipocyte dysfunction in metabolic disorders.
- Primary adipocytes from murine adipose tissue are vital for investigating adipocyte development and function.
Purpose of the Study:
- To describe methods for isolating, expanding, and storing murine adipose-derived stem cells (ADSCs).
- To provide a protocol for inducing adipogenesis in cultured murine ADSCs.
Main Methods:
- Isolation of ADSCs from murine adipose tissue.
- Expansion and long-term storage of isolated ADSCs.
- Induction of adipogenesis in cultured ADSCs.
Main Results:
- Successful isolation and expansion of viable murine ADSCs.
- Established a protocol for long-term storage of ADSCs.
- Demonstrated successful induction of adipogenesis in these cells.
Conclusions:
- The described methods provide a reliable resource for studying adipocyte biology.
- These protocols facilitate research into adipocyte dysfunction and metabolic disorders using murine models.
