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Updated: Jun 6, 2026

Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
Published on: March 7, 2015
The generation and the manipulation of human multipotent adipose-derived stem cells
Brigitte Wdziekonski1, Tala Mohsen-Kanson, Phi Villageois
1Institut de Recherche, Signalisation, Biologie du Développement et Cancer, UMR6543 Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia-Antipolis, Nice, France.
Abstract:
In this chapter, we describe a method to isolate and to expand multipotent adipose-derived stem (hMADS) cells from human adipose tissue. We also describe culture conditions to differentiate them into adipocytes at a high rate. This culture system provides a powerful means for studying the first steps of human adipose cell development and a route for investigating effects of drugs on the biology of adipocytes. Finally, we provide a protocol to investigate gene function during proliferation and differentiation of hMADS cells by means of siRNA-mediated gene silencing approaches or forced expression by transducing hMADS cells permissive to infection with murine retrovirus vectors.

