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Updated: Jun 6, 2026

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
Published on: December 20, 2021
A dual-emission Förster resonance energy transfer nanoprobe for sensing/imaging pH changes in the biological
Ya-Ling Chiu1, Show-An Chen, Jean-Hong Chen
1Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
Abstract:
A dual-emission nanoprobe that can sense changes in the environmental pH is designed based on the concept of pH-responsive Förster resonance energy transfer induced by the conformational transition of an associating polyelectrolyte, N-palmitoyl chitosan, bearing a donor (Cy3) or an acceptor (Cy5) moiety. We demonstrate that the developed pH-responsive nanoprobe can be used to ratiometrically image and thus discriminate the pH changes in the biological environment at different length scales.

