Analytical methods used in conjunction with solid-phase microextraction: a review of recent bioanalytical...

Area of Science:

• Analytical Chemistry
• Bioanalysis
• Separation Science

Background:

• Integration of sampling and sample preparation with analytical instruments is crucial for efficient methods.
• Microextraction techniques and microdevices offer convenient solutions for this integration.
• Solid-phase microextraction (SPME) stands out for its simplicity and effectiveness in sample preparation.

Purpose of the Study:

• To review recent applications of SPME in bioanalysis.
• To categorize these applications based on the coupled analytical instrument.
• To highlight advancements in SPME technology and its coupling with various analytical platforms.

Main Methods:

• Review of recent literature on SPME applications in bioanalysis.
• Categorization of SPME applications by coupled analytical instrumentation (GC, LC, MS, etc.).
• Discussion of new SPME coatings and automation strategies.

Main Results:

• SPME enables direct and selective extraction of analytes from complex biological matrices.
• SPME coupled with Gas Chromatography (GC) remains the most popular technique.
• Significant advances include automated SPME-LC, new coatings for direct extraction, and direct coupling with MS, ion mobility spectrometry, CE, and chemiluminescence.

Conclusions:

• SPME is a versatile and effective technique for bioanalysis, offering direct and selective extraction.
• Recent developments show increasing integration of SPME with diverse analytical instruments, enhancing automation and applicability.
• The trend indicates a growing utility of SPME in various bioanalytical workflows, expanding its reach beyond traditional GC coupling.