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Industrialized MS-based proteomics in the search for circulating biomarkers
Isabelle Migneault1, Joanna M Hunter
1Department of Protein Analysis, Caprion Proteomics, Inc., 7150 Alexander-Fleming, Montreal, QC, Canada.
Bioanalysis
|November 19, 2010
Summary
Clinical proteomics uses mass spectrometry (MS) to find disease biomarkers. An industrialized approach is crucial for reliable discovery and verification of protein biomarkers in clinical samples, overcoming biological variability challenges.
Area of Science:
- Proteomics and clinical proteomics.
- Mass spectrometry (MS) applications in biomarker discovery.
Background:
- Clinical proteomics investigates proteins in human disease and therapeutic responses.
- Mass spectrometry is key for identifying and quantifying disease-modulated proteins.
- Clinical samples present challenges: high variability, disease stages, treatment variations, and sample handling.
Purpose of the Study:
- To review MS-based industrialized proteomics for clinical biomarker development.
- To highlight strategies for overcoming challenges in clinical proteomics.
Main Methods:
- Focus on mass spectrometry (MS) as the primary analytical technology.
- Emphasis on an 'industrialized' approach for biomarker development.
- Discussion of standard operating procedures, process control, and chain of custody.
Main Results:
- Industrialized proteomics enables reliable discovery and verification of circulating protein biomarkers.
- Standardized methods are essential for managing clinical sample complexity.
- This approach addresses variability and ensures data integrity.
Conclusions:
- An industrialized, MS-based approach is vital for successful clinical biomarker discovery.
- Standardization is key to overcoming challenges in clinical proteomics.
- This review focuses on the practical application of proteomics in disease research.
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